fluorescence single neuron and network analysis package (fluorosnnap)  (MathWorks Inc)

Journal: Scientific Reports

Article Title: Lysosomal and network alterations in human mucopolysaccharidosis type VII iPSC-derived neurons

doi: 10.1038/s41598-018-34523-3

Figure Lengend Snippet: MPS VII neuronal activity and MPS VII neurospheroids calcium (Ca ++ ) imaging analysis. ( A ) Ca ++ release in control and MPS VII neurons, upon KCl induction and reduction of Ca ++ signaling upon inhibition with nimodipine. ( B ) FM-1–43 fluorescence decay in control (black) and MPS VII (grey) neurospheroids at day 28. Data are mean ± SD of 3 independent cultures. ( C ) Percentage of cells per number of spontaneous Ca ++ events in 300 seconds for control (black) and MPS VII (cl. 8 grey; cl. 13 light grey) neurospheroids. ( D ) Visual representations of neural networks from control and MPS VII neurospheroids reconstructed using FluoroSNNAP; circles show the position of cells in culture, yellow circles represent functionally connected nodes and red lines represent the functional connectivity of pair-wise neurons. ( E ) Ca ++ events peak amplitude, rise time and fall time in control (black) and MPS VII (cl. 8 grey; cl. 13 light grey) neurospheroids. Network properties: Connectivity index ( F ) and Global synchronization index ( G ) in control (black) and MPS VII (cl. 8 grey; cl. 13 light grey) neurospheroids. Data are mean ± SD of 3 (control) and 4 (MPS VII; 2 with MPS VII-cl.8 and 2 with MPS VII-cl.13) independent cultures. Asterisks indicate significant difference: *p < 0.05, ***p < 0.01, ***p < 0.001.

Article Snippet: Image analysis was performed using FluoroSNNAP – Fluorescence Single Neuron and Network Analysis Package, an open-source software developed in MatLab for automated quantification of calcium dynamics of single cells and network activity patterns .

Techniques: Activity Assay, Imaging, Control, Inhibition, Fluorescence, Functional Assay